	   SnapGene      $�ACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCCTCGTTCATTCACGTTTTTGAACCCGTGGAGGACGGGCAGACTCGCGGTGCAAATGTGTTTTACAGCGTGATGGAGCAGATGAAGATGCTCGACACGCTGCAGAACACGCAGCTAGATTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACTTTTATGAGGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAGGCCTCCAAGGCCTACTAGTATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCGAATTCgccaccatgcccatcatggtagatgacatcatgaggcttctgtgctccatttcccaggagaggaagatgaaggcggccgtcaagcactctggaaagggtgccatggttgcgggggcaatggccttcgtcggtggcttggttggtggcccaccaggaatagctgttggggggaccgttgggggcctgttaggtgcctggatgacgagtggacagttcaagcccgttcctcagatcttaatggagctgccccccgctgaacagcggaaacttgttaacgaagccatggccatcatcgggaacttggactggacagacgcagtgcagctgactgctctggtcatgagcaaccaggccatgcagcagaggctcttggccatgctaacgacctatgtcaccaaggagctgcaggctgagatccgctatgaggacggtggaggcggatcaggaggcggtGGATCCgccaccatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaaGCGGCCGCAAGGATCTGCGATCGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACGGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGAGGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTTGCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGTTACAGATCCAAGCTGTGACCGGCGCCTACGCTAGACGCCACCATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGATCGAGTGCCGCATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGGGCGGCGGAGAGGGCACCCCCAAGCAGGGCCGCATGACCAACAAGATGAAGAGCACCAAAGGCGCCCTGACCTTCAGCCCCTACCTGCTGAGCCACGTGATGGGCTACGGCTTCTACCACTTCGGCACCTACCCCAGCGGCTACGAGAACCCCTTCCTGCACGCCATCAACAACGGCGGCTACACCAACACCCGCATCGAGAAGTACGAGGACGGCGGCGTGCTGCACGTGAGCTTCAGCTACCGCTACGAGGCCGGCCGCGTGATCGGCGACTTCAAGGTGGTGGGCACCGGCTTCCCCGAGGACAGCGTGATCTTCACCGACAAGATCATCCGCAGCAACGCCACCGTGGAGCACCTGCACCCCATGGGCGATAACGTGCTGGTGGGCAGCTTCGCCCGCACCTTCAGCCTGCGCGACGGCGGCTACTACAGCTTCGTGGTGGACAGCCACATGCACTTCAAGAGCGCCATCCACCCCAGCATCCTGCAGAACGGGGGCCCCATGTTCGCCTTCCGCCGCGTGGAGGAGCTGCACAGCAACACCGAGCTGGGCATCGTGGAGTACCAGCACGCCTTCAAGACCCCCATCGCCTTCGCCAGATCCCGCGCTCAGTCGTCCAATTCTGCCGTGGACGGCACCGCCGGACCCGGCTCCACCGGATCTCGCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCAGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGAATTGACTCAAATGATGTCAATTAGTCTATCAGAAGCTATCTGGTCTCCCTTCCGGGGGACAAGACATCCCTGTTTAATATTTAAACAGCAGTGTTCCCAAACTGGGTTCTTATATCCCTTGCTCTGGTCAACCAGGTTGCAGGGTTTCCTGTCCTCACAGGAACGAAGTCCCTAAAGAAACAGTGGCAGCCAGGTTTAGCCCCGGAATTGACTGGATTCCTTTTTTAGGGCCCATTGGTATGGCTTTTTCCCCGTATCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAGCCCCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACGTAATTACATCCCTGGGGGCTTTGGGGGGGGGCTGTCCCTGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGCATTGACAAGCACGCCTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCTTTCTAGGGTTAATCTAGCTGCATCAGGATCATATCGTCGGGTCTTTTTTCCGGCTCAGTCATCGCCCAAGCTGGCGCTATCTGGGCATCGGGGAGGAAGAAGCCCGTGCCTTTTCCCGCGAGGTTGAAGCGGCATGGAAAGAGTTTGCCGAGGATGACTGCTGCTGCATTGACGTTGAGCGAAAACGCACGTTTACCATGATGATTCGGGAAGGTGTGGCCATGCACGCCTTTAACGGTGAACTGTTCGTTCAGGCCACCTGGGATACCAGTTCGTCGCGGCTTTTCCGGACACAGTTCCGGATGGTCAGCCCGAAGCGCATCAGCAACCCGAACAATACCGGCGACAGCCGGAACTGCCGTGCCGGTGTGCAGATTAATGACAGCGGTGCGGCGCTGGGATATTACGTCAGCGAGGACGGGTATCCTGGCTGGATGCCGCAGAAATGGACATGGATACCCCGTGAGTTACCCGGCGGGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT  O!   =   /      5   -   4   /       6      $      A   %   +   +   +   &      4   7   /      (         !   (   (            3         *               ,      +   (   !            9   (   $   ;   3   (      %   :          :   ;   6   -   /   (   &      &   &   *   +            &      2   (   +   "   2   0   %   ,   =   S   ,             #      !   #   !      $   %                         "   ,      #   5   !   %   #          /   !   "                     ,          !   '   9   '   (   "   )      &         *      !   )       0                  ,   "            %   &                    %            )   *   #   (   ,   C   &   '   !   %   /          "   *   *   (   %   '   &      &      #   %   !   -   .   "   /   9   ,            *      (   #   ,   (   <   P   &   %   $   7   ,   .      "   )         1   '         1   8          6            !         #   3      *      +   <      !   L   (   %   )   &   +      #      >   !      @        = ^ Àš›�‘¥Úë./08Õ-.defpq
Yµ¶RSTY€¶·‚ƒab4ãä !"WXpqyz{|}~üiëŸ Û#/ §ÁàhœZtÛìù19lÖøVgål
Â
Z·þŒUŠ¸å#Y]E†¼D<jìéÄ ”!3"ž#k$ $ _ ü €¶Á
Zj�²ìë' 5;¼ÝZý  E$5 >RôüBmz’¦å¼B/rb	¦	ñ	ŒÄ	ï-Ÿ’ž­ø¯„º2šcú‘þ>r	“1˜!ô!Ü#s$- ¨ï8ùW†÷®Mo{Ò¶	Ç|D“–V¹¶>\ƒ™ñŸ¢ÃSVRÊ!E$16ZFk•!4 œÂÜáií:üXÅæ’ÂÉÿuR[ÿNáKY½ó‹9¬«MyÝ>‡æ=÷¢ê ° ð!4"Í#T$l$|$/ 7 �uú2Â×ƒu„W
c
m
å
J‡–Ñ†¸ñ÷imh'É	ƒqÐN½ãEg|íÏ¨ Å à Ÿ#ì#  .[ÜmhŽíòh"	Ã
�¤dÁNÚ.H”æ,^×Vv„ó6 ‡uñ S@L|l�	 	
ú
ì>Ï	' kx³g�©¬KKz"=g6Š¡µ¶‹£$9þsÂØR y"U#F$ ·FÐzÎ‚„åš[½Òb|¡·þ+ L Œ"$ % ` ý Â¿¨É'HøH	
š
oalë=íG�ÕA†4<n½0sÚU ¡"Ö"# �±²Zî
Ê+´[•ì(C×+Þç¨¨¡ Ì (!"ã"A ?SÊÓõýCn“ª·½CJr~°§	AÅ
ð{«&~“–ÏAhkž75@d¸æ$?Rÿ>�”�p€¶Ï5v•ò¿±"}#“#Ý#t$% Õ “Ðæô{®0L	ò	9�._rŸ°»3ûf
9”: G!q"#X$+ Y§áC+Vãc	�qÇÒŸËùÿD¶:–kpŸÀÿBs€êÎc°C2æ™!Î!‘"+ Ë Y{á@I	6OsÛ2«æ7 N®Ž’…`›w‚š°ÃÜ'’�´^)Û¥!õ! #+ …½XXßú)fp|	Åj³)”W°5œbŒ¿ 07T"ˆÛ[.LTnqµE!–!& ö Ö‡øZÓì+•'}`ÖØJsº™?H]š#YWSqFÏY…‡lÒ ¾!n#ü# úŠ2	·	µ
�—Kc„Äl†ØC‡qË& !<"´"X#„#4 3 ©·ðè9œËh±¯NÕºÈE2·AJŠÅìyòc£µçóU‹Ë+gÈ%27G‰gá¹T±ù!I$7  ÝâjAî§ýÓ“ÃéP	´·S\ãèO‘±¨ìLuÂ[:\­‘ÍRÑ4ˆðç£ëÓð ˆ !ñ! "5"ö"U$/ N t Ï BÃØåë–n;Æ§;	X	†	2 LZ†°¾ÍôŒ¼Ù^Þ˜À�?kq½>øy  w µ Î#à#}$ C í Ð¹Yg	u	à	£
vÜâY¬z—Yåp#m$…$( (e�ÍV	u¾çÊ 	Ê	Ú	*
Û
—*f¼Ùùqz%~üNº¦ü>± ¶# Vž5>Dv£Ô „Æ	x
¹jnS’¿õ©º[O~ähD#$ 8 ø r�Õ3Ãî]	¾
Kˆ�‡¼ø
Šó¾F}îŽÐ†!�!¶! #! ±­cØªX
d
n
©
S—¯ÒÚ ©3`ºiÊÝ„r�,©l Æ Ô Õ!( qû~¥\v…Õ
æ
-òbÚø_šÊÙì(ÕsäÑ5 € ‹ “ á í V!«"½"ë"í#þ#"$( ½ÑÞ€ ï9‰‡÷i–Ž”!PV¥Æ3Ee4N��/2‚_naWQd¦  K\Ý'×�ó#	Î	Ä
É
dÂÔOçì&5Ø3w€�ô-!w$ /nÎî—Â¡ºá±U¯Rš…†v!Q# ò d�i>T©›Þ"¶×ØhÛIòH•¨-¿PÀ!€#3 ,Ñ	òÀTAMŒm‚ 	
”?ïÐf¢+y´e��ª­ïIL�Á	Q%>Ó„dŽtðÖÙS ® "w" cæ3~¡s„	Þ	%(�× ów˜ÈL×$¤…»
C!O#V#G$ '§M¡	
û
[!#
œÿ˜#h·Œ%¹ñ6�!z"#u#* y ˆv¡Öè }ý.
¯
âí!lÉhen{7‹¢¶êø ':ÿH®8Ã¬!Ê"Á#$ 
GÑÎ·	¼, M ƒ!B" ‡ �¸×˜Hy&ÏC{ƒ…b³í óÓ"c}¢ !>"ù"@#$ G ¶R‰}	í	ª{Áæ ››«Ô\z¾¸øÿ!�"w# ƒ^²O4	{
À«š^ÆnŸZ#, þ e³Ô(IamI	£	¹	¿	
]bÔì¦9<Œ™OHˆô�51d!/( o!-"_"b"Ç"ï"ý"†#ù#‚$ & � ˜ ¤ ¯ ‡xÍ¬	>&B«Ú¾‰ÃÛ ¿ 1!M"¶"×"	##+ xÃý)©ÀÝ©Êü n˜
›
pz¿?žî‰úÿ.‡õ=oEu²t8˜V ^ !T"ˆ"?$( a ó“�ùD		
�
`m×ÝR†�³j�Ö
Ö«¯ÅÞ¬º#GÍd!E"¢"Ô#$! ‚#=z	u
ý
ŸVËo\•ÑM–ùÒ)Yã…ÚÉ9E© ~!…" 8Ì�òzE…
v'íy½,èþ¡ðèN©mÍ |"ò"4#f#–# , J²á[ï
¢´µô=Dé§ÅØ¹ßl¢ )![!"" H�³@	·
,9Dx§ü“‹DU„^×s"ä"9 5 òxØ¦ÇD+Ãs_È‘ñ~L”—Bi8Ié,;A_e�%i4@UP7Ì7<‘gq´»ÐÀP Ô"{#( @TËÔéo”«š·È	QæR„CŸ2.‹ÍâSl,iR·w³T!¼!²"~#Þ#u$$ ‹ ¢ F1–Ô¾Kª	Pvl–Æ›±WÑçÔüŸžx-6–î”#=$; f öþ.7D]êì¸±¨	Â	äBÆ�4|#¬'Ð0eò}È
6|ž¹-/O™'w ó?•—$e�¢°óW r û!X"©#3 ;S\”—Ñ$|¯eúÿ#&SM	�	¼	ú	/Ëªá{4 58g½Ê¬)nô•˜®f i t ý!##³#( Ö _¦!<1×
0
:‚É`çä­ø£üV­N‹û; b ±!Þ!"Z"Ì"K$Y$c$  £ÃÝó	#j²Tt¼~¤Tky0›Ê:\õêQVr"$#*$% h ’çõ	Ð
G6s Ç1s±ôú#Z¨Ì£¾›	:gq–:H!#$:  Z�£P•^±»PW¿Ï/	d	¼æéÙ Œép¥·õ— GJ�ÇüVYAd¯NŸ±2.3T I ®!ö# ø<„_Œ•³8aäS	¡rÈ/úE�‡;ÐyÅç8"k"  ¹0ÿâD	8…Õä {·Dq8 tÏ™1‹Èõ:!š!’"G#: ¸ G¨­ºjê	,å	9
 DŽÓí �®Ìèôl÷&ÁCIëÊ&ƒ“¤ÄDg¦² ' / © Ç!Ï!�"œ"Û"‰#Ã#; Ì ŠÇ+9žÍ
�	$36üÔò)8DW|Èg¯�“˜œ«®·8Onƒ›»(1)I!kã*Åí!ö!0"#`$6 { QŒ½âŒ¿"ÖÜ3Š™³±
'ïevrçõ;6Wî5éSgè„ÃÊßÝjì‘µJ_Ž› é!I"e"9#«#- |î AJ‚7tý	ó
Ê[­n¥Zaj¡¹Ú)O,‘Ðâ±=¸>"šøÛYÜr!"/ Z«`m¡áY1ÇP™´¬óÄ	#=%,SÒß†atxÄÍî9Q�±t“ú9Möì-IL¦!�#( # ] û =Q¦ßûAl™ÀYs�
k
Á
´îX±�¬1™ùÿ:�\»êÃ —!ó!j$r$& ÛîöûWLgzÆÐQ…h•cÀ¡8UÉ“LÜÏMUöƒ§ò~ ß ë#S$ †¾ðY{	ù
kyê*Î³wƒ”¨!‰3mµ/Šr¶& Ê X©?HIà*Uq}ªâ€Æå6Î�¯#¿³oÎ(B”¾F!p"°"W$& ¶g°ù(Ôë´
*¹i(_Õ×1r@I›Äb6TÇÚ„†ˆBmp°Ê% ;"* M ì '×äUm¦¦O	t	¶ÌëKt»ø$Z}XûÌ]Ð—¹€–päûïv ‡ ÿ!õ"o#µ#+ ÷ =CbqŒÔ}©Óÿ¤[Åí	,®ÙaÙ÷^¾Øër¨ÜòZ+�k Š Ó ì …!Ô!C#ý# ñ &ˆö•È
–~“OšÁ2×Gë®€¿! x år‹üƒ	Zá*�Èdc#ð	Õ­ B!«!"#t#À# GQ]~±3	ì	¿zªÍ]Åm¶÷!!="A"ø"Y#& w²Üû’¸	¾	Ó‹˜Nˆ…‡%€ªÙDÄÝˆÌ ' !0!n!D"L"S"µ"ü"…#$ ËŽàt
¶
žU³uCÐLø<ÄTçï8lr!"e#2 4 Š èñé0™ÓÆ¶Â^åQ3BKÅdèV^Œ
,hÌÓá&3TOk6hò6Qºí²O S!ú!Ó"<$(  ‘:–d%ÖF©Æãós¬Ë¢MSjÉ9ŠúéU—­e h Ý!#²#)$J$+ · ¸�^i²]°OR	8
» „ÿ�‹z€C¤¶ô7ÏHÆû®MÃ¥ÿ-. 7"›"Ú"" ªÆ_l�Ìà˜Õü	É¸ÇÞí+RsÌ°is8ëâ÷Kš è!ì!2 <¥ÚÞík˜¨ï>GK©á
ø
P„égÅ²äívÍ§ ®Èþ’‰Í
'ñ} Â Þ ò!¯"ê#V$i$q$0 ë &ãBp‹¨þ”¥ÄêÇ
µ¸Øh’N^Ëêö½Ã×aÖúÛê\Æ–ñƒ•AúŒ$ † ‰ B#% w v±ÛFßYàTÒ²¾yŠ©MŽÇ‡\;S©µæïkÔ!!"@"÷"û"d#¿#,  ‰ �Åçkc—ÔQ	7

]äPŽ¨r]¯LiÎÒà8Nú5ñèö¤ì, d 6"Ò"= v ê %;�¤ÄÙæì—oŠEj=¤¨
6
÷
ß	ÑÄM]‰±ãõ��ÌÚKh[Åßåð‚@îõÌù	&‹# | Ý !Ñ"¾#h$S O P u › Ø é CDÙš%&'()*/07M<œ�‡	

 
!
"
2
3
4
5
G
H
I
^
[\‡ˆ¿ÎÏÐ™äïl$%]^! " x y z { ¶ Û Ü û ü Î"Ï"Ð"½#Ï#á#M$N$[$e$f$g$, Q Y ‚ œ § ² Ð Ù ä —#›þ8><	m	#
J
@3Ð½ÑG[d¶šÁÝcr¾_ö ý 	!\$~$  U EÚ+1NÇžY	ˆ	_
¿EÀ…� _m?z= A · "!ã!Ð#â#O$ D 4$¹õ ?3«=	×	 
w§>˜·d˜0sVþ 7!³!]$$  R Z � Ç î ÑøûZh	¤
HPeù›¦ÂÈ�Õà!P"q#™#£#n$†$# ƒ � ¨ œËÿ9{º	“	á	$
K
Ì
)4Ýb Ò\­À{dÍ¤¿Ð÷ )"(# ³ Ñ Ú å r˜u�n	v	?
<AIãÑZ¾ÞöÁ¢Zæ`
!!>!! )5ÎºÛ
Ð;Z	Ë	Û	+
`
XgÚé{>æ
On bñ² \"¨"Ñ## V W,J(¿Ÿ	é	F˜òú}úº;M¯D¥`“»ÅÚý¤> P$! a2O+ÈèÙ‰	Ü
+Á†rìýî)�§eà¸ #!‰!"·#ã#$ Ã FfžgvËòÀ€½&XÂ/ý@{?wB ä!`#$$ W6jžºö@Î%4¬Ù�Ç`	ï	U
Ško<Zö€ª?�<åtz¦�"##% 1 ?VbÕÝ…dçV	Ø	¡
Ù
º1T´Àf¦¸¾ÖP|1WÐ ÿ !´!»" E Ÿ%¥w!C>	y
x’¨7A“‹ò™¥»\ ºÅ"	$€$ 5E”W¤Å!6		›	%XGúe™�”i8!7#E#y#^$ [ È ï ù ÒÄ¤[¿

¬‚Q¬@ýl!Q"r#°#  S â sŽùüCa¢ßÕ	½áÅ<If‘¤§ÃËôìï‰‡!á!&#¡#o$  = ‘ y¸Öå_	^	‰�ˆÃú‹œž ¢ÉŽÕG!! 9 ó4ïi	¥
Lù¿~ÝÖ�Ñ�!·!š#¤#‡$  ) „ {�ì6	â	Í
5aÞcÞ2®|—2^À|*"h"Ê#"  "‡®dÈØâ"|T‹˜L
O
e
Óª4»jËÁeÎ-ISªm Õ ø &",  ? I l “ © ¼ ²ÄÌç:Ùï¯	Ñ	õ	%
o
ª
3T‹˜°!*ÜÓ]mü|�¥úÇ _!Ö! ž MT÷ «»¶”	Y
EeÛø¤wè{…s‚Ñ—")#i## Ò ™r®ºü’â	w	³	£cù:`V›¾ßätÑ×ó[ai‹t"ì"5   ´ æ Njsv‘¶ËÚßw&	o	@
R
Ö
=Bj}�ËÒl8Ú¿"V•¢É®÷ ÌÒ÷£ÀçXØ !?!Ú!¬"#Ç#! ƒ¦Š
�
ç
%róèýË)�Ó�ß³Â � ” â !N!W!¡!î##$,$% Û Å�]†»C
.JäÛ>Ýjví[Öï­åj^ÆÞ^6 Œ î h!""¾"<#ÿ## ¾ßé÷6ÓQja
mIWhê|ý?f5€x3Co¡XØä� ’!œ#z$ ÏÜˆÑ<[	Ì	§
0�•Q¦F!`ŠŽ0ePßR<„³ �!©"  *6aÏÒû�…‡ðÜ	,

mÛòñuO‘ƒ`±ëbsL½ ]"Ò#/ Š»NZ¡:Š“ø—ñ-	Y"Ç!BÓ-43çÎ¡ÆM <ªo˜còep § Å!"! ?.]Þ�Àô	r	ê	AýÃAPª¦JaiÛ4â�¥Èõ.!n"Q$" W 	-K)	k	Å
Ê
ÖGeÂ~»<ÕpÎEYbè”Æ6ÎØ[?  ; j ñ`�´Ø 	$	Ï	ˆ
›u€û8VEâíh¼xÛþ¹"®# %LXë(}
�.­™óûHN°Í'ÙÖ‚êx$ bïÚÒ
ë
N’‰‡œsâv€VS¨*—‘ž!#1#R#$ ^”õN~˜>»ï°†fáìŠ! íPowÉé)	6	›,	¢\*)‡$!"¸#3$   03Ï,Š	Ý
ƒÂÃíþ‘²›È¹ w!#\#ä#$‰$, ó GwjÌ
Áûª�­º¾œ#o„³Ñì²$iÏÜðÀàv|–.-	ÌQF ¹!Ë!�#¦#ó#  Ä g"›ÂTH�h
U9·ÉJÈJóö0yÁ‘C 4!ž!å!a#9$ ~ *ež?“
1½Øåö%IY�Ãï¸AÓ è J!! mŸ'hçó¯õßu¢'PünÙ‡¥¾©þ€Œ|À@xÁ!$$' ~gµ-k»÷AUcoa	¥	ð	‹‹,Q‰µ‘÷®¹&ox»Cû~0D$9 -
7Á…‚�­ìñnƒ‘ÁÈÑþ!	V
ä
t•ðöl{àCg£µ=[‚ðMžÂ
RG« DÈª‚=fu{¡¯ Ì#' ŸÏ&GK÷
í
ë=fŽ«JÔ ä@…Ö*™æ§ ×ý* K T Ë '!"x"‹"#T#( XÒ’óz­5BNÚ1K	8@pÑzž?�™·Ûežþ�bœ4uñ“Ú¤!�"ÿ"#" 2  çWÞYe1	Ä”&4JëxŠ×Xf†pÑ !D!W#m#û#H$)  B d@Ït½èW	f	…	Ù	ß	)
¢
Ú
³â°Û)eØpyÁ»Ø¿3¥¼XÒ  !„$ 4¢Ô
Ž¨»2_¹‘ô™É¹‰}€4 ’ µ!¼"!$& ¼ŸcÍî8œÖ†c¨ UÝ!µÖMg§à°~%4Q`2™P¥ ,!u!P#  &}¦ç®
“î "$›¡d$Öƒ�&¸º57É"N# F † 	ÖµxNz
©B ™šò»‚!v#
$* — ® d(¨ÿ—`wÌ¢	
\_y¾Ö;>�ùŒió-ô	ª±Â¹] ,"a"Æ"#ø#�$ + "D?	ü
8”¦Š¦¼C¸ƒ]DÖZ!{"ñ"3#! 6\w•×¥*Á	PãŽu
/H|ûž;#w¡³¯V W"z#) ¥ R#þ"RÜ	€	»	
/
Ï
Ê0rùYz§÷£4x7¼fšÉ[•(P9a °!
"Ë"##$  ¢Fé7	ä	è7ìãçèoö%IUX1Ä 9!­!j"F#ˆ#Â#0 z ‰XÆ!~³œ	°
ò
&îdu"mñViÙRf8Œ·ëùL!H j ,IÄq!/"d"8#Ž#_$ \ É ú úŽHzÀ
‚�ºA
½ L ð µalÓ¥¯Ò­qÀ1¸îë „!:" P�qÏÃm!C"R"s# Åµ\ƒRrSÅ­þ¬- N Ù"±# ˆ °ÚbàXO½Æ†¨´~Á ?"ú"A#p$, T � š ¦ ± ã $Ž–£Ø‰™ý$D£]
ö
¾ÐDâ„ŒJgÿZÜãîbíô#Šõ ú !!!!â!¼#" � q3t�Ê÷úzªÖ	Ÿ
¦(=øc¥ÌõÀ/¡UÔ=!("O"'#¢# Â ¹fI'Ï:|	Cí(’ÄŸðd£ßˆ!$ UViBcæU	_	~	š	î	‘‰06@eñœÕ¹!Ž"#x# á ·äB^`Ô	«ÂÄ;��Ÿ¡£Êˆ!!%   ( > H ’ !†zÃÇ×áæ!SŠ—®	N
2Š÷£ç1û{�H{ù^!%"&  M­¹º	²	Q
$|Ê!œ¡ÈÒ¬Œ­Ð²Ö]Å¿Ý]hŠ  × M!Ù!"#  `‰„~5†Pð,	¦
lñ,ü _‰ dÿ©KÞ×ão œ Œ!‘!›# : >-êð_€³	j	|
€Õ-š¬tÁG@oÍDgÇém"¸" ôMv5	ê
M}ˆ›=0#[#2$ˆ$ š 
g
’
ú¬¹0¼äƒÇ½,xÀ‹�ËÒ 3!�!¸!¥#ò#  }f´y¬FJbnë0J	¤	7sßBP´˜w›}ü) Ê &!Š"þ"Ë#%  A ÿ »žÎÌí7›(
�²á^b§dI‰ß¯}32_eoÑ3 ‘ ú#ƒ$ * … – ­ Õ|þË­
^Õí:=š ¥øÕ,‚‰Á\ +"`"È"ð" )	º	À	ã	
Î
6cë�ðØæõ3$6G"v0` p!."V"c"i"‡#�# K ´ŒpyÂqŽ~«Ø") Ž ™ ¥ ° Á #2¯eˆÉÙ#©Îž
W¥¼‹ì'÷þÛâ¿ž.TÀ ô ù !<!'"N"»#*   ' à ¬ÂÆàã TUh™	­	±	Ó	M
P
#5?0: ¬ÏÄÜ\‰Ö Ø!"
### ˆ,éï}™	+	f
|Ô,«‡k¸ÌþCf+¨wJŠÊ n 2!œ!·"/#ñ#1$( @ • ¬ | Í«Ëýš'
¬
6rŒ±àAŸï¤×÷ÔˆvFuû[ _ � É U"‰",  J À ³+«ÁÅßèSo	˜	°	Ò	�
V{"+Šý/öýp�}§v�ó !×!"º#0$C  	 ” ª « ½ ¾ ¿ yz{Äþÿ*ªÞRª™
	&
œ
«
45UqÝÞß@¨©áâ ünopqŠ‹Œ¢£¦âãä%Ñ9:W X Y Z — È ò !I#/$& 
 m Å;qÚðö	
p
™Àªãû^r>¤‘¶å&Ò³3;™Þ÷˜ `!J#5$@$' Ÿ N�¼:8	E	÷	

‚
»
š äü‡FŽå´ktƒ¬Æ­æ$ a!F"à"Ø#$!  Uôø”�	q
ÍÜ Û}¤Î(^Ós_s×’°Hå e!”"˜"&$6$% <?¬ä·•	Z
Áùÿ:¥x�ÞS@aé|·ßøÎ™ *#K#j#Õ#A$/  b n 	ÆrÛñžú
FafnØ.«,¹”‘×@ß?†¥òæ'Óß´Ò4‡�<š»£"  Ó šs¯>ãF	´	…WÌ]œuänÛ„:\¦®ç á"Ù# ƒI»	9	v
¼
›Ä	—aˆWÓåZ†b$" ñ$l;x	{	
ƒ
 ¤d–ýG¿úÒØ­ÇFª¬!jb!!u"ƒ"†"í"$*   O‚ý“€½ø	þ
!pú;ÒåN�àæ,‚©µ*lìEuôÊ‘�%ŒG"* ! µ OV{¢xr
Î~žÌÓ(’ÅÜ~)ö#ÊØ!Íí±éøOÁènI« @!Q!Û!™"ó"'$(  ç 9t5·ó•‘	ç	>Ýwê9¥_ÔÀ–´¾-Ã“¢Êi„ Ù !­"^#—#è#% / º õw’�ÌÛàA
S
�
×
C¡ßÛ“£øÿÓñ¤ªûÎ f!•"#5#g#Å#È#‹$' kÍùÑF'	p	†
k�mÏîtzW`tH¯é	AÌ(•ÆY» æ É!}"#7$& ³åâ
ð
Â;s¦ôy·TEÆ¨Ô(º 	 £ *!X!¢!L#k#B$ K@„\­¸[
�
è
&M€ïAb}ÆŽ¸Ãš !\!#"&  =§.Ž	–	£ žéþ¶ßõÌ> êžà´à…mÏ1 • ã -#‹#Ö#ï#$$-$ - âŒ	‹
úKµÎ*êÙ´àù‚ O!+#æ## c ª¯´.^‡¼A	G:K¢y¨Þ•×LàçÔàÇˆF¼Ø_7 "ž"è"=#% 
2DÜä�¡ì
D
bgoÙåï?Ðký!ŒKÓ5ß=)ti!Ñ!"" $!  sŸû¸
ß
/ü-Üm-w´ØAù@E‡__µŽ¶� ï "Ý"å"- o Ü IÇ‚†¼Æò;
–
FÕ/E¬]°ºî\”’\Ûð V¦®æók…(›¨ây!¤"¿". ¿óy¤Ùäêø7Ô,äXÃýiëž�ê`3âý=VDÜ…Y’;hèž “!2"�#Ú#" 6 ›Ätµ	b
I†MJX’¼ò˜&Ù-Qx+05pÖâåÎ ï!{$/ t°±§ÈER?k€G	Ÿ	™
n`ÉÍ<&FJy<BfŽ´¢#8r§¼ÑÙa{ÁQ  "Õ"â"9 Ô Éà¶Ï¯^ªÊ%Mq}•þ@Cgj‘�µ69_4v•Âå&joAQé8Í=�]¯µå9 Í!|#’#, õ „Õ¼›Ê‰	œ²I¯
˜Gb‰"Xa‹¾´/kæòp…*f‡Î-KSà¸S´½!ø!³"ƒ# s Î AÌê•¸Ò:	É	1ç�–…§¯[�Qjïx=´ ß# U°p¬\	w
¨
½
RRÔãU!Ž!ª"ê"$ ÐÝJ=Í	SÅD Ó3Œ�¹1ñ�Tmc¾O…#v$* +bÐ2Õ¿LÝ	
-
eþ—Ö€òmv—ÇîHœÀPÒé÷„G­ï€!v"$ Œ‚—ˆ%|	Àžªa²ÓìŸy!?#( Œ £ Óòü†mlœC	«	
€
¥8ÜQþ²Õct® «.7—"F¾ c!^"‡"î"Ó#>$# 7IG€ñy<y	„
¡n&w~óû’ÑXâèx„ý ÙÈMk}!„"•#, e ¡ -Eë©	Ç	Çƒ�}"O•1šñ-Š°Ç	+:{�Ð.N˜v+Ë–df,q »!¨#( g ^[¢Â;ùù	"È
5æi±Sà"¢}Ÿ½Uš¬/«pômós ü!Y"#b$< ÷ÿ/;ƒO‹¬¹þ””º`¾Î.	ÿ
åCØ­Ô.†óFx@˜É%0‚Š’ž£f±Çô&S H ¨ Æ!€"õ#P *8P‹¼í 0�‹¾‰˜²òÃ	Z¬#25qû<¤Óô$(+:CY`{(5VÑ4èf�—Ïáç~ƒª­¶ÂÉÞ7Nmºí0<P~(=õ™HÚX�"H"ª#& " P@W¿rõVTyj
ÏxÍ“«˜ø29Kl´¾Î²éõ‚“¦o"R$% <T%f|£õ'	N	s	³
)Ô0÷|ê5§ËÙ­*oão¯Éj u Ó!þ!ô"´#$ Êä\}°‘û‚	½	ë	s
t)BÌ÷$b"îÃÜ7ö ¬ /!A!ª! "K"#$7 ¶ Ž/]^•˜ÒßÁ $û	ŸþÆÝÄâ*BQr«Ê¡	6hËjêù5PLÂJ–™g R!Ü!ç!ë!š"#($;$, u§FÞ–JÆ
×gƒfxÊé§¼¦Ö`ÕqùÇý®é•7M”ùj+c … ®"c#é#. X × è :".6Li
=›l	

1
F
?ÞÃËÏFZcŽµÄË\< @ Ú Í"L$Z$d$  Æ 
¸ô2	’	>
Ë
;HaÿO—¿ÇŒ£Ï!6!²!ß!˜#" 4`*Øè	ñWñùèë¹:=Lå®¤WÁ.üÙav"["§"_#$) 0 “¤a�ÄÍÜ$Ø 5�T
Ø
$;Y³ WŠF¥¤½ùœ;{“y¥ÿÏ º"Ä"#6# < xò£¡Þ		�àÔ«Üë?ük!%#¯#  k » LSöë5îµÐ	ô	DdÛvlz– 1]Rg"–"h#É#1 iÄ‚‘µŽÊÙÞá%	B
‰
Ž
iqœ¢çü9=k7UÜiu½ÞãU”îiËòöWg! !"s";#Æ#+$' ÎúMYèöÒ’~
l/H AÒð2tÍwÅL
°ê
;Br—;ƒ¼ Ä!~"y$ i $	q	‡
7@üU©ÌáXaIhÚÍÕ×áZ­# ]“ì(	Ñ
‚‘š[u()–Ç��ë# #$1 } l!&)æÁSGŒ®ô8nt¡ÈIO²Ðëõû$m±#†¤Îï¿ßõu{œî·E ç I!Ê!$8$8 ‘,„�ðÀÐöýã
ì
ê<õzŠˆ��¸F˜¶ÚCŸÇã©Õ)�nýºBaeú3 /’ŸJ £!S#C$  ¼X0	e	Ó
Ã“§¯¸Ú3oÜxÕêºˆW˜O ¤ +!t!l# $ c´–æ_v½cóB‚©°·C¸Õ4Y!2#M#÷#6 [¤Q"²QÛñ
OçtUtâ	Qç/qø.y¦öw»HKÈTWZ+O ²ÃU 
 ¯!	""# ù`k�O®´p�¿0~R¹Äíê 9" € p•¢ö9\
õ
'NBYaÞ!  L…A–]Ab¹åT	1ö¢/ÉdðÇÑ�z±‡gL!]!$"! _=Lu…ÿ	‘
é
™s/»ð+ãûF‚ˆ<?¼cÆâè› ‹!l" ºxEÊê/]ê³å+#šnÐ2 %!Œ# ß 1îg¨*	¤†·ë9Ÿá*É[ˆ;!›!.#ð##  Q p		—	§à£Ööû‰¡uµá$œÐ2Ýö� – ñ "H#¹#.$4$3  €>ã97	�	º
Ìþ9œŸÿ-Ú
+|£¸Í'E]“Òä??`rÞñ†Œå ä “"ß"×#%$ ðHk „Ã+�¨ëmD�œ¥«‚"$* . ¹ 4Ð¡æ	Œ
�Þ‘Äéõ’³GÂ@ìË'”ÅÉhúƒ ª º P!È!#]#Ä#ç#Š$  ~
-‹	�	á
JL¶ÍîÿÅ'„³ 0 ,#Š#å#+ 1…©®»Cã- ë:
•
Þ
ûEÔî¡\l¯Ï ³K[øÚJE§µá(x!Ð!"�"Ü"ç"< È£°µÎã®ž	˜
HÂü;L%»E�©É$p�´%^ØP”Á2³áwü*/<èÕá
"ŽäÍ`z8 Ì!î!1"Ÿ"‘# r Í ô ¯É±®H®½Ò³j¸ño„ð)€�Jî ~½N÷!é"‚#! H‹xk›B	
7ý%¿Õ}í�Ðáw©èöƒ E|!>#L d ),:ŠŸ«Á/¹_ˆ½ÍÆ	«×14‚	£ó'*9X_zUh°R…”ß!–™ð‰¬¯ÁÝ9zœ¼Ï;MT}—«.*lÈ/‰+GÆÙRG º!"#§#ô#a$( �¾Éãq[ôUi
²
(wsöqÉé—÷1k³Â6K’ ©!Ò!æ!ê!"J"$:$% Å 3hEÝ	Iš
=
E
ðfVðøè¦¸ÊKþÆ�Lø`*u5!¦"b#$) hKRœê4�¢ÃÉí#×Ý4Ž´chpÚæ<:X6ÿh‰Ý•z’>j!Ÿ!"f"Ã":#& | \ #å‘ÀR‹?@Ñ7ïêTl"v…ÄËàKÞôí¶Ôà�œD Ã!¬#$+ !+ƒu€õü®2bnÛáPwÔæ.÷-xµÙBž¹òAm‡¹`¶ùBNŸ·ÂÔs!"  o¡jKt@þ	ô
0õ.o.º¾*âcXQ`Ýé K!#  Þ f8¹
Ëý\¦¢²Ù*&’Ñã>_úÝ"ˆð#›ÜäZ Ž ð Þ" }ï„BŸ ”
à
IÝ[k€§²JZÄF?�ª"æ"> q ®(‰¢ÀÈâš¸­Å	—
°Ö0$G­&^TQ¼ ï]ˆÀy“Îï:SŽª²v¨çõû;)íÿ‚�.}DMy{!�#! [JÈgpZé3ÈóQ<
÷>¥þpˆÜu–Å”²÷¨!Â!¥"Â"$$ Ý n iƒôôH-±àvÓá)b¦^Üñ!WPl†Mž©Áã@ p ¬­abcdefno‡ˆ½¾¿Çè2µ¶·GöF£¤à(»ýo‡ÚÛ•¿R]u§¯°±ô:NO�JKLœ�ABCz!§!À"Á"  5œ  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        5                                                                                                                                                                                                                                                                                                                 @                                                       	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
      
                                                                     
         4                           /                                                                                        3                                                                                  (                                                                   	                           )            
   
               	      Q   5               
            	                                                                  (                  ,   L   9      +   ;      (   +   <   )   £   n       )   #      +   )   �                     5                                                                                                                                               @                         	                                                                                                                                                                                                                                                                              
  
                         
   4         /                              3                            (                         	         )    
 
     	  Q 5     
    	                      (      , L 9  + ;  ( + < ) £ n   ) #  + ) �   ‘c  “  “?  “‡  ™™   ‰  ©o/Udï]œô :‡ˆ‰ŠÇâãóÝ_ž	
¹
0IÝ$%o­®.^¢¤¥ï…–™yÁ÷ÜÝ"ˆõöNOPQ` ÆÝv!ÿ(™�%Î#…#PQA8»í!$>èÖ*C¶	“\Êkõ›¹
IÝo.ˆËé.0QoGSºØA¦¾Œ"#0
æRü4Ë#;aÌCj—Ç–#2;t®
8e—»Ä(›¤Á ‘ì•ø$x»-i í!•¸| @—‡çFv!À!‡—j•2çFv!—çFv!�–s"#y$µõµ|øÕ(ü± ü#@ÎBƒ“\Ê|ÎÃhþÕÕ
Ù—ŽvÕ
òÕâ
Í1 Ù#áúõ2é�ƒ·Âì±Ù+�0q*ƒ„�ƒB{¢è"ˆ Êá "$p–lûAûA*à Ó	:?H¯ï�‚GK×Ôà\W"åö3	Y#‡|°ì kg&rŸÏ°5¸6ŠµþG!«È3 ðJç‹g2¹Ú\"Ñ#àÚ1‘�™Û&�]ÿ"œ § þ8¹#
Ñ[¯ ÌÚ|'%$ðtï‰–°!&3Ï�5¸æ‚“#}
þNNhèEÅòG8Á#$8$¢Èâ°G­¼û;„‘È-S&"8	³å!ž¯„7‹‘ø8$Ú1Û�„©¦hŒ„Ë‘7G«$°&Ï5¸“‘=#›«\7 2J÷	½|ÏÔ
‰‡ÈZ¨!=Q"Q?y»Æl"õý·°A«&Ïd5¸
&ÿ>€ò¤` ‡œ¨§gq|„°ÉøÇìlÒ kŸg¶&rŸÏ°ƒ„…5�¸ÊÕ6Šµ‘6þCG!¥!Î!¡"„ÐzCÐ!nhƒ ç#pê"×™F¾!`†ðt°ê¾pq¢£â+Eœó#×IŽô
i
±®²:X½íÿv–—9Æ÷2áu²û:;lmJ¹ùž—y¯G:Kº$×.ÔàÌ_ž"1±!g"ÇÍ�	$DÈ#o�³À	!Ë!ê-•Å” TÞ
‘&p·A.ýÉ'/qø¦pÉ‘&§Oê"#‚žè‡œ#×Ž:Xÿ,©¦åhèŒE!žÅ¯ò„Ë7‹‘ø7G8Á#$z
/ Û×
Î þEœ#×IŽ²:XÿÆ ~�	ÿ„é·ÂÇò xŒ�êšÍÔ ³SÄ	#%ÈäOS1l�,Ìx—Í-9­½	C:›ü–‘•-J·2b é!œ#×Ž
:Xíÿv9Í&£#VE›þ†¤[„å
,ñÙë' Š à ì ì#ý#T‘9Þòú�	#u) #¢âû;9Šýó !º#rð–.‡¢y•FØ"hGŠ„Ðz¯C ÌÚ|'Ð!%$•$–g ççHTW
 ¹™Í¦&,,cMsŒÍ 	{
zC¦(¢ƒ˜×L	&,u¤8 !$/ Í­Ûãx×
â
GÎôKy¬m—W_Ô,×EÆ¨“Í„Ôà:çmÏ1 „ Î Ñ!	Ë
a2ï¼tÇÍÈ�Ž¹Á ½Í×þ95LRG ô#nƒ(Iö!mQ"Qy»Ž þLhnƒ ç# � ù�_Óò h€#$¯¦á"D$]~±3	z
¿™mY#Â p@#­úu„å
ñ'Ð à ì#\…-ÚìÕä5 ‹ í ½"þ#¾q+ƒôU€õüi
w—µÙ÷Bž1k³m¹`ù’Ÿ, á´éØ‡•}™F¾!Z£P\”—Ñ±ÿ#Pú	æpá¥õ 5gÊGJÇVYNŸ±T•˜f ®!#Yz²Ohr«+Ÿ­„…`šÚHwÃ”‘’,¥!ô!"ã"¢£âã­—
Âü�]“2²ávûü;<�y1"�# 'K£U#F$kFmÏîtzæ É!7$²h+ÚH”,"ã"\”—Ñÿ#ú	á 5gÊ•˜f #Z£P±Pæp¥õGJÇVYNŸ±T®!h�ÉÝhpæ<6hÝŸ!":#V5Æjnõ©äß|ý?f53äŠ
6üÔ|œ+9Z£P\”—žÑ±ÿ#Pú	æ3ò)8WLg”¯Bip“á8˜¥«®õ8› »%i5gÊ@GJPÇ7VYÌ<kNŸ±´*ÅT•˜f ®!##{#`$¾q¢£âã+ƒôU€­õüi
—
wÂü�]—“µÙ÷2Bž²á1kv³ûü;<m¹`�ù’Ÿy1"�#ÃjTt¼TÊõVr"*$D£	hD#ïë
‰‡œâ€‘žR#Éã[sÂ6 ©!"J"$�uƒ¸imNãgÄŽB
=Üiuîig!";#¡‘Äõìª P!X0	Ã“3êWl#ZÛlgÂ
Œå]H�D“U„GbQwj�jê	
mA¾ìxÏàva¡¨ÛØõ.!1#¡X0	ÃN“3‘ÄêõWìª P!l#äû‚	)b"¬ A!ª!#Ñ
‘u)–�� # 9‰÷–!Æ3d¦ FvWçüŸ”#(‰À¸Å	Ö0&^TQ ïˆÀyÎ:Sª).òxØ+~é_U‘g/Îªu	X
µ
�ÚâKÂY±„r�Yšåqv!ÐÝ<y	nÅD3Œ�Ñ1Xâ�„mÙÈc}!„"š{¢´	…Â¦·ÓÊØn¼ £ *!è"ó"k#8¹
ËýÙ*&’Ñã>_Ýðä Þ"Ýjîèìu­‘ˆðñ!U$¸Ì~H&E…
{ LíyBè$‰c¢ 1!|"¶"O#$âA§ý“Ãé´·‘Âˆ  =Ž	 žþÌ> …ã Ö#$$µ
�Kq/ÎÂ±šv!Ì~E…
 Líyè$|"O#ªu	X
ÚâY„r�Yå+9ž3ò)8WLg”¯Bi“8˜«®8›»%i@P7Ì<k´*Å#{#`$äû‚	Ñ
‘)ub")–��¬ A!ª!# #?çV	Ø	¡
Ù
À¾Wÿ ô e!”"a
IWoz$A	=#e ¡ -Eë©	Ç	Çƒ�}"O•1šñ-Š°Ç	+:{�Ð.N˜v+Ë–df,q »!¨#d ),:ŠŸ«Á/¹_ˆ½ÍÆ	«×14‚	£ó'*9X_zUh°R…”ß!–™ð‰¬¯ÁÝ9zœ¼Ï;MT}—«.*lÈ/‰+GÆÙRG º!"#§#ô#a$5 òxØ¦ÇD+Ãs_È‘ñ~L”—Bi8Ié,;A_e�%i4@UP7Ì7<‘gq´»ÐÀP Ô"{#ƒ^²O4	{
À«š^ÆnŸZ#· ¸�^i²]°OR	8
» „ÿ�‹z€C¤¶ô7ÏHÆû®MÃ¥ÿ-. 7"›"Ú"f öþ.7D]êì¸±¨	Â	äBÆ�4|#¬'Ð0eò}È
6|ž¹-/O™'w ó?•—$e�¢°óW r û!X"©#ù`k�O®´p�¿0~R¹Äíê 9"qû~¥\v…Õ
æ
-òbÚø_šÊÙì(ÕsäÑ5 € ‹ “ á í V!«"½"ë"í#þ#"$Ë Y{á@I	6OsÛ2«æ7 N®Ž’…`›w‚š°ÃÜ'’�´^)Û¥!õ! #È£°µÎã®ž	˜
HÂü;L%»E�©É$p�´%^ØP”Á2³áwü*/<èÕá
"ŽäÍ`z8 Ì!î!1"Ÿ"‘#0 “¤a�ÄÍÜ$Ø 5�T
Ø
$;Y³ WŠF¥¤½ùœ;{“y¥ÿÏ º"Ä"#6#· Ë Yª¸ÆY{�á^_l�§Ìà@Ii²á	6CO]s˜°ÕÛ+2OV«ãR	c	ü	8
»É� q„ÇÒæŸÿ7� ¸ÇËN‹®ÞíùÿDz€Ž’¶+:CR…¤¶`s›ôw‚–š°ÃÌÜ'kp°7Ïis’ŸÀÿ8Bs€HÆêû�´Îëc®â÷M^°Ã)C¥ÿ-2KšÛæ . ™!¥!Î!è!ì!õ!7"‘"›"Ú" #0 < á x“¤·äòBUVai�^`£ÄÍÜ$Ø 5Bc�¡Þæ	U	_	~	š	Ô	î	T
Ø
	$‘«‰06@�àÂÄ;Y³ WŠFe¥ñ;¤œ��Ÿ¡£½ÊÕùœÔ;{“y¥«Üëÿ?ˆü¹Ï !!!k!Ž"º"Ä"##%#6#x#¯#Œ$*6aÏÒû�…‡ðÜ	,

mÛòñuO‘ƒ`±ëbsL½ ]"Ò#¥ R#þ"RÜ	€	»	
/
Ï
Ê0rùYz§÷£4x7¼fšÉ[•(P9a °!
"Ë"##$ƒ � ¨ œËÿ9{º	“	á	$
K
Ì
)4Ýb Ò\­À{dÍ¤¿Ð÷ )"(#}ï„BŸ ”
à
IÝ[k€§²JZÄF?�ª"æ"…½XXßú)fp|	Åj³)”W°5œbŒ¿ 07T"ˆÛ[.LTnqµE!–!Ž ™ ¥ ° Á #2¯eˆÉÙ#©Îž
W¥¼‹ì'÷þÛâ¿ž.TÀ ô ù !<!'"N"»#~ · *6a†¸Ï�Òû^�…¾ð*e‡ðYiž²]{°?O	R	Ü	,
8

“
ù
»ky ê*„Îm³wÛÿ1ƒ�”½ò‹Øåñuz€¨ö%C¤¶O‘ô!IYƒ‰�Ã7Ï3mµï`±ëHÆû/bsŠ¸L®AMÃr¥Óÿ-¶ . ½ è J!7"]"›"Ú"Ò#ƒI»	9	v
¼
›Ä	—aˆWÓåZ†b$  7Q     	H  $•        $•{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷Øý¶	vØC®#½«ôPÃËS{�P#Ø(À`úË:
?}“Š Ê >)øq t­OÊûFé`N?üátÁ^Š\SØ=¶À^�àtsHÓ‡Žà¶_CØÓD; ö9qù‹}A8ÐáÎaÁ´Âào=öõWeECðÏk›ÎÎMxÙC˜QÏà¯�u£ªtcvlBméÎzÅbò° ÑNa\f…�[t[,˜Ò{ºÖº˜ðŠ2š›£J3ó©–!hít˜«4ùò¬«ÕP
5Uª#UTïÖÛÕVšm�®UÁ§Ð´±‰Ü¬·nÛŸ¿
¥…œjˆôKëÔu£xm…ï¸ž�•³¸ûd^>2®Í�Doš¨5µm¯xÆ©H¢½¯mÂ¨*yÞÁš†b¬K.–&F¡EŠ¸A>´uÕ#8à�_æ»ë¬õS{†‡Vq©¹jw£®3¿îå,L(õ¯n=	´5`;xG�´æ¸ißÄ a•ð3	RŠöª…1Bˆ�ŸW8¥µD1xˆ„Bù†˜¼)‰]o1õÏ¡¡Ô9ÚàGdéàQÅÞºÛ(|øRîRuC›Büáhöèï]ÿ]ºÇ4"Í¯ÇsÂ¯Ï<¢Š MÁQÆQ iË=-IuóRŒÁ¢* qIñPº|{ÔUmí•Q@ÁQ³È”ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  ¿  ‰  �  
  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *õ<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="1600014C10Rik-m" directionality="1" translationMW="15087.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3851" maxFusedRunOn="3851"><Segment range="1734-2156" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="20" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2193-2202" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2199-3851" color="#ffcc00" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4413-4422" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7368-7384" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7344-7360" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7437-7458" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7392-7398" color="#ffffff" type="standard"/><Segment range="7399-7416" color="#ffffff" type="standard"/><Segment name="-35" range="7417-7422" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4116-4384" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9296" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8505-9296" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9297-9365" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5828" maxFusedRunOn="5828"><Segment range="4443-5105" color="#05fd14" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5229-5828" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7746-8334" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3892-4103" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5936-6057" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5225" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5828" maxFusedRunOn="5828" detectionMode="exactProteinMatch"><Segment range="5175-5228" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PBÂ MinimalÂ 5'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6548-6857" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="PBÂ MinimalÂ 3'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="870-1110" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:17:59">2025.7.16</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  70     	@  $w        $w{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷Øý¶	vØC®#½«ôPÃËS{�P#Ø(À`úË:
?}“Š Ê >)øq t­OÊûFé`N?üátÁ^Š\SØ=¶À^�àtsHÓ‡Žà¶_CØÓD; ö9qù‹}A8ÐáÎaóß`QÏUvTT?ö¹¼àQ�|àD×�”9…œþ
øZ:§F7fÄ vÞœá'¬V/+
æÆ`8YÕ·E²É�!W»­k©� 3)©°J4£?1qÚ™arŽ×I€Ú³OŸ ŠÊ½U £UZ¡r5UNým½Ui¦Øå\}«K�ÊËví¹ûðªXYÆ¨�D¾±�GZ7†Ø^û�¹à¸ùP‹;�¶Eãã!ìØôFùªƒ[VÚðgŒj”Š+ÚöÜ*�b§�ì¨f jÄ²ébdjQ8«„ëA§Q­R3Žõþk¾ºÏU7¸hug›–§qZ:àƒ;þîPRÀÂ�Q
öãÐ›CPf·„yÛNk†�ü@_0°•(¯j¨S(�¸ðs€Ê[TCˆˆD/˜i‹Â˜•ÖðS\úA�ƒ�®vNžÅ]ë­²‡Ï….á¥'T9´ ïÎ�onŽõßõÛ¬sB,Ðºüq‡<!züóÊ(ªÜaÁU²œ³ÒÔ—_1U(ÌJ"¢Ÿ§ÁW±ÍEVÑ¾ÙPõ<‰L<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  ¿  k  r  ì  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *Ü<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="1600014C10Rik-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3821" maxFusedRunOn="3821"><Segment range="1734-2156" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="20" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2163-2172" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2169-3821" color="#ffcc00" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4383-4392" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7338-7354" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7314-7330" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7407-7428" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7362-7368" color="#ffffff" type="standard"/><Segment range="7369-7386" color="#ffffff" type="standard"/><Segment name="-35" range="7387-7392" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4086-4354" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9266" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8475-9266" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9267-9335" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5798" maxFusedRunOn="5798"><Segment range="4413-5075" color="#05fd14" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5199-5798" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7716-8304" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3862-4073" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5906-6027" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5195" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5798" maxFusedRunOn="5798" detectionMode="exactProteinMatch"><Segment range="5145-5198" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PBÂ MinimalÂ 5'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6518-6827" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="PBÂ MinimalÂ 3'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="870-1110" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:17:44">2025.7.16</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  5�     Ð  "Õ        "Õ{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o=öõWeECðÏk›ÎÎMxÙC˜QÏà¯�u£ªtcvlBméÎzÅbò° ÑNa\f…�[t[,˜Ò{ºÖº˜ðŠ2š›£J3ó©–!hít˜«4ùò¬«ÕP
5Uª#UTïÖÛÕVšm�®UÁ§Ð´±‰Ü¬·nÛŸ¿
¥…œjˆôKëÔu£xm…ï¸ž�•³¸ûd^>2®Í�Doš¨5µm¯xÆ©H¢½¯mÂ¨*yÞÁš†b¬K.–&F¡EŠ¸A>´uÕ#8à�_æ»ë¬õS{†‡Vq©¹jw£®3¿îå,L(õ¯n=	´5`;xG�´æ¸ißÄ a•ð3	RŠöª…1Bˆ�ŸW8¥µD1xˆ„Bù†˜¼)‰]o1õÏ¡¡Ô9ÚàGdéàQÅÞºÛ(|øRîRuC›Büáhöèï]ÿ]ºÇ4"Í¯ÇsÂ¯Ï<¢Š MÁQÆQ iË=-IuóRŒÁ¢* qIñPº|{ÔUmí•Q@ÁQ³È”ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  Ð  J  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  )�<?xml version="1.0"?><Features recycledIDs="21" nextValidID="22"><Feature recentID="20" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1745-1754" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1751-3403" color="#ffcc00" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3965-3974" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6920-6936" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6896-6912" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6989-7010" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6944-6950" color="#ffffff" type="standard"/><Segment range="6951-6968" color="#ffffff" type="standard"/><Segment name="-35" range="6969-6974" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3668-3936" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8848" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8057-8848" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8849-8917" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5380" maxFusedRunOn="5380"><Segment range="3995-4657" color="#05fd14" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4781-5380" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7298-7886" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3444-3655" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5488-5609" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4777" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5380" maxFusedRunOn="5380" detectionMode="exactProteinMatch"><Segment range="4727-4780" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PBÂ MinimalÂ 5'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6100-6409" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="PBÂ MinimalÂ 3'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="870-1110" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:17:12">2025.7.16</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  4Ž     Î  "Ï        "Ï{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o`QÏUvTT?ö¹¼àQ�|àD×�”9…œþ
øZ:§F7fÄ vÞœá'¬V/+
æÆ`8YÕ·E²É�!W»­k©� 3)©°J4£?1qÚ™arŽ×I€Ú³OŸ ŠÊ½U £UZ¡r5UNým½Ui¦Øå\}«K�ÊËví¹ûðªXYÆ¨�D¾±�GZ7†Ø^û�¹à¸ùP‹;�¶Eãã!ìØôFùªƒ[VÚðgŒj”Š+ÚöÜ*�b§�ì¨f jÄ²ébdjQ8«„ëA§Q­R3Žõþk¾ºÏU7¸hug›–§qZ:àƒ;þîPRÀÂ�Q
öãÐ›CPf·„yÛNk†�ü@_0°•(¯j¨S(�¸ðs€Ê[TCˆˆD/˜i‹Â˜•ÖðS\úA�ƒ�®vNžÅ]ë­²‡Ï….á¥'T9´ ïÎ�onŽõßõÛ¬sB,Ðºüq‡<!züóÊ(ªÜaÁU²œ³ÒÔ—_1U(ÌJ"¢Ÿ§ÁW±ÍEVÑ¾ÙPõ<‰L<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  Ð  D   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (<?xml version="1.0"?><Features recycledIDs="10" nextValidID="20"><Feature recentID="19" name="luciferase" directionality="1" translationMW="60644.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1745-3397" color="#ffcc00" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1745-1748" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3959-3968" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6914-6930" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6890-6906" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6983-7004" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6938-6944" color="#ffffff" type="standard"/><Segment range="6945-6962" color="#ffffff" type="standard"/><Segment name="-35" range="6963-6968" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3662-3930" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8842" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8051-8842" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8843-8911" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5374" maxFusedRunOn="5374"><Segment range="3989-4651" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4775-5374" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7292-7880" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3438-3649" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5482-5603" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4771" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5374" maxFusedRunOn="5374" detectionMode="exactProteinMatch"><Segment range="4721-4774" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ©<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:36:2">2021.4.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -ö      )  Z         Z{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2306-2315" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5261-5277" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5237-5253" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5330-5351" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5285-5291" color="#ffffff" type="standard"/><Segment range="5292-5309" color="#ffffff" type="standard"/><Segment name="-35" range="5310-5315" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2009-2277" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1752" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7189" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6398-7189" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7190-7258" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3721" maxFusedRunOn="3721"><Segment range="2336-2998" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3122-3721" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5639-6227" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1785-1996" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3118" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3721" maxFusedRunOn="3721" detectionMode="exactProteinMatch"><Segment range="3068-3121" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:35:53">2021.4.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Dý7zXZ  æÖ´F !   t/å£àÐ¢] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Üµ¡&õý{ÍD¹Ü‰G+·,®\9gfÝ+<	eË[î^0 ÌntBŽîz�Š.bÌ³šZ•&Å+îáýÄ© åê�°ÅqL;œ×(©mX¸0ÂQñ^N�á]öÞ'Š)x håÀ�MÑ›Å(3¹ j|ÿ~¨eš_d»@®M¯ªf çK”SaàU"M–¸Œxþ{'…§muÄœ®TÜWMWz}Ö�}!D:Tƒ´¸\<K©‹s×!à„�óVXœÔÚe{r ¾JÒ'wr¥�«õ�†ùséö›äl®‘Œò¦Öãçõö9áÏß" Ëv
�D¤m2ÉÏ0KµM­÷yŒZ�íC�‘8†œð@ø:³e…�A?kjNV•Óù~‚§„-i-«ÏäÏŠŒ››Ë‡}ëllaÆÜñ±LîúŽú®‰øMð½7Q„õJÅDÀ¦EDOW)h(8£2)˜QÇ™ïð"FC©Ï%ó/}JÆ‡:Õßzn¾C…lb»é]tn4%F,ÇðoýàuÝåš´dÕšx�ìkÆe¯óA›wK ŽM‘xšÞÉ,æéü‚5ò;�- ßúSé@1)W�`èˆw÷ëÿO	ÂCD"D1˜ŸV^ìFƒv—eÑí¾‹{$tÝ›fö(�$ý_ÂØšÔÔò|Qx¥s�rw:J�Á~}Âê7žv�ÈHÅ8»”#ÃK	M\�’kø8s,®ò…èLZoGQbÁ8ñÖpz�œÚÚïLíxB	à°ƒu1­€š3uÂ©‘JèfÆÁM3í­¤¾«æâVhæSaTmOY8×�vÐñìYÝ�:Ð×�|-}Ánz?êhÒT×//%N_ý™‡#æN.z|åZJ–ü ó@g§­˜ Zðç+÷Î�%ó3�ËÒç}"ÅÑ.÷‚¸ÅûLv¢»Æœ= �ùò¹#;p7¯"z¹&E¡¢õ©Õßob¸ª¿µï1EÎþðÀ†›(“mŸ“ÓåÛ¯¼{åiÑEYöz;n^ß2«;6<#ý|qJçE«LnÏâÖ]€_éPI5l3Òt‡>JŸ%²¶‹qAÉ’$?¹8Ëb·9sHÖZccž7�Ô¦í%!ø<WœŽ›ãùdåÛÖ�[,í·ëjv^Ã:jÌt�_%0ÿ­p*Êátdùh&^âš¬FÞÝìµ_±dÁ‡#S Áçñc·Z3ß´¡¢N^¤é^-G'‡ZÙl¶½ú,5Û›>‡g5>$æ>"¢€«�Œ±ü-4;+‘­çõöƒ—D‘3#ïºâªf—â˜F¾Ý¡A;§˜2Â­|;úÊØùmN‘J)ôÌ“-óµpßÉr“6°Mà=¨ñj$…¿ÔüÛ´93®7ŽÍõ—ÙÃ�ÊæÇõýõÃ~(=VÔ¿ÀU‘Ñ°ëˆŸ½-üdm`x"Ôý/G�¨w^Vúµ®AÜÁJy›ä™I¼1}Ø›PüŸˆ¦�ú�1+›§ÌÛÙéhTõ1²L`ôŽ|ŒQ©·8ý!+É›&ÉŠ÷£¶ßFäûÌ„Áõ5m�©é@ø£)ŸƒåR»Žð§Äwø>Í�½GBýältx7`€ä»p‰NTÃ/n«z	¦€8IÞVM<3µë‡ˆï÷"Ÿ‡šv
ˆ2R'F†¸ó­lô.Œ&Ð÷d¥‰õo/mHAÁ-¤OPqIç˜¸ØM%×¤C›‘Ù©=”jf,—i‰rÏáÊÍÿ« ÏYjÛþ\y˜|''º˜vr¨Œ,ZXf?¾†]_´&:…ð8Óû�oš‰äDh[;Xk…<Ùù¸ÉÐ­^­CM4ÕoŸÂnB~þ‡Ã`'3Ç¸ýrà«<]†§;ŽÎyi`b5­`a”ÌMÃp92þ^F�hVƒí´ëo¿ý}½Ä*È[ÏM	s|fágf7µ2«‹^fov¡áè`V•à#Âª&·Â��âÔB�XX’í�2¹U”™Ì­éÕ‚- ð†¿­ù;¸[7Oá Óaë-f‹õ’Ê^C³ÛµQTü-ñi0Õ÷2ÚÇèÛOéO±åÔlfŸÒsu� ªtvú$oq™æ¦_ý‹ð‚MKyìbrÏ
ÓÈ€:˜Rš|]Œó*ñõS’æZfõæV¿œŠ*üh3¨™…
Ù~ô‡ÒÇû³>"�	©DÎ´†,Û‰wSG7Rèó ƒLç‚´ž
¨‘¼6 Oþ‘kb8‚˜Ö[ß�º–„/�|^¹ÖúÌâ¡Ëó¹D~ãÀ$m Åôe|`ô
ß‡=OcDM	‡¯cs&¯~�i>. ðn‘�0?ÞÇ©†q®ákŸ¶¥ñP=ÇÕ9fæ7`\ÔËà£1Š(ßé_!7�*r Ö¬è6
n¼Q"Œ´°Ù†ÉliìêÿÞÝq×—@y�PÏB)ó‚þ‡Í%è¯¢!0‘Ï*?8óaÛNM*¸<Ž+�¿w'P7ç†8ãa•–¿ÀÄÌØa¶âÔ\O×çø}ÉÏáÿ¸AÞ&Ø¬uÜ3Ö7ïÕžWŸ¢Û3ÀºNlýê[tãŽ]òÕ%þàCÂuÒ’—»+¾
rb2¥�ÑZ}?‘×M=×E€'#2‚Ú:Ö°O1ÒryC”spû.d¹AÅgÁaWfð3@çd!>õÓ¶XvŠs5�l¿^drÕ‚eVš�7	Ê²—éËénG‹ŸwÀÔø^V‰û2 ®ûéƒ,t¸—k¦ˆ™¡ZMy0ŽÄ24	'BÔ ú186îžDÒÉ¨ô¤F8¨aBòx_«wì¤²V¸}� 4P‰?vÒ’-‘¾`Ez%¹mŠcÂ¾ö¾”)�Àˆ;µ·@û<QPòzä½¶©·3>®´G®ñý˜Ñ1"èà²à:Ètm=ÏBÎ,®R—dWT6+ Ò/—¦{3Ù' q
÷8qñÌÇY8´¤BÊ¹1ç¸É¬Ó�~_ö¡z’´öb¢~N&\ˆmÛš (•¬ô¡¦2Öª·ysn7O�”	>?…—äWBõá-¬®PÁÅµmß’®0Xšê—‹–Wémq�Õ1§îKC»=Ó�Äh³_“zâ¯2ÏïgãÂÇù�-ÅÓxg��+“†Ö9Â‘J‚Rå‘ÇŠ.¦†2„:|�t¯r€>Œ«:‡ÿ»‹.ÁŠ
”;øâNZ	Á$šðLÌv–ï”çÊ†«b‘ÃœeLù/ˆ@Æ¡n�­ö	ûÌkú»)ž¤tº4ýtÞÈu!	ÕüèüOZ@�ç#-¬q*©6*º\'?ØF oŠÚäßLÚ7»´Á-ÅøŸ×€„<2cÁ@Mu¾wº[Ÿ;6åÖn�×é:ÐD3É!òí4ÕLVEÖ¢®<$äWMáø§s/õ–V4Að»ó�E– ¢ƒZlHÙ£ó•qÃŸ]Su€}OðÏog;Ex:í]ÿ¦ñåÈTFDž}¾Û9²@”û«M\…}Ö³OÒ«=ÙoW‹6àUÝ‰_RªæBÞ£äsí§Šr:ö ]
A�Âõÿ öŸ£vï×ÑÍ€À&ÂÊüêd]ÆxÔ�é¸¾äÉ°9-]X³s°ÂµÍÁC¸•ÓÖùœiž+1|.DüùF˜.Ì5”ÐVR\ç¸­Œ†ûógëë�Sð”mœ§<º#Ý%è†tKÿƒ<	;1ÿ�`YäSJIfãXŸV*@Ò2Çu‡ÁÚ;«áùqS#Æ¾Øù�Ÿ¯—ß]žØ…g}7¶ŠÆ¸úzac*?xk¢°Ê/@È§#nµ—£M€vëÎ�OCº?ýi+ÂRü¦ô`~ý1žE�T&ˆ2æuóÙ#U²ÑGE› ŒµÊ´k¦r=Rê¤'}%
Áj7âJÚ<Ü-FUºäÜ¹qKk¹ùhÃg,©^ó±²23i;vxèbqq§fz«â´˜’PXƒ×nÿ+ôƒä¥z€�UZ>äŽtÊµ®ÆœTe›Ç�ð¶®sp]™>wË¾Ñúf®RJ›ØP©^½#èüc\C€cgqAQ!�D¬6ëF�EI´ÄElÜ­F~ì„€äEb5¦áÄYé©ªHÛaÇà>ù˜ÍùÓÞ_^9 ñ
k
!Ú6I|Ì…ÊÕó/Š>æ-Hêøˆj&È¿¦EâÊbðbG„á¸ÇFW">Qaf7	Ò®÷�‡¿�—y ùp�ö5x©œ¨G=Ômj�T¯üÀX©ucb–7J?jrÖQó0úH{wH×‰"ÙJž(˜f—%(^Lö²Nµ-o‡Ú ó¥²f�Že„ªÍ«zÜ6½¦Ùçé�^º'Ò&:–_.§q]4>h¶É·ñ´4>A†"Ÿ³;Ë„HS–þfeã”ˆœ„C¬CÒ:¹daš/­‘¾e•Ã'1ó¾LÎ?Ä ùak®1HÎ#¼«wlMU*ãJêÓÉz<€>ÔI
nŠ»2=ðœ~!û}Õ‘ASÞâS±$L$k�¸�¢ÍÁ�¸·�œB°„G'�.û:aiÑ�˜Ë5:dâÓ|¸¹*›/—‡¿3¬õxi¾m¡Ì‡Ìœ¬½&Åêy6=‘Õý~ ß>S„_ìO9g íÝ3ÚÄ®ÄÕ>í€4ŽeVÃÐÖˆám¿n?tÙwÅ›ÀN%Î~¦“Ù;�MŸEbÓ!šuããÓ­ÆI5¶}êo­-wX«Õe7V_Sæb+À¹ÆÿFÛÎ´aH‘O8XJ¥0�EÂ²Ê|ÐpÉ{'ÜLnÇpãy\ã’ùõT1cpf�ë´Üžp�™ŒÄVÔY]�JDíÃå‰pT¿5wr!sƒ¾G µA‚aLöïTX*T-Äïú«\à¶”·ÅPJUM›š´¥´§2h96Ò4CG_ä¤!é!þòâðÛ 9Sòøÿr¥€N…¤ý     wîœéwg �£¡ Ü*4±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0»<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="0" name="2Ã—Linker" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3845" maxFusedRunOn="3845"><Segment range="2157-2186" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GGGGSGGGGS"/></Q></Feature><Feature recentID="1" name="1600014C10Rik-m" directionality="1" translationMW="15087.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3845" maxFusedRunOn="3845"><Segment range="1734-2156" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPIMVDDIMRLLCSISQERKMKAAVKHSGKGAMVAGAMAFVGGLVGGPPGIAVGGTVGGLLGAWMTSGQFKPVPQILMELPPAEQRKLVNEAMAIIGNLDWTDAVQLTALVMSNQAMQQRLLAMLTTYVTKELQAEIRYED"/></Q></Feature><Feature recentID="2" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2193-3845" color="#ffcc00" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4407-4416" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7362-7378" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7338-7354" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7431-7452" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7386-7392" color="#ffffff" type="standard"/><Segment range="7393-7410" color="#ffffff" type="standard"/><Segment name="-35" range="7411-7416" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4110-4378" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9290" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8499-9290" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9291-9359" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="11" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5822" maxFusedRunOn="5822"><Segment range="4437-5099" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"/></Q></Feature><Feature recentID="12" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5223-5822" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7740-8328" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3886-4097" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5930-6051" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5219" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5822" maxFusedRunOn="5822" detectionMode="exactProteinMatch"><Segment range="5169-5222" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="20" name="PBÂ MinimalÂ 5'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-6851" color="#a6acb3" type="standard"/></Feature><Feature recentID="21" name="PBÂ MinimalÂ 3'TR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="870-1110" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  À<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac-CMV-1600014C10Rik(mouse)-Fluc-EF1a-CopGFP-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="4:45:50">2026.3.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  P              E              ÿÿÿÿYô? P                                                                                                                                                             »<StrandColors><TopStrand><ColorRange range="1900..1900" colors="red"/><ColorRange range="2044..2044" colors="red"/><ColorRange range="2125..2125" colors="red"/><ColorRange range="2156..2179" colors="blue"/></TopStrand><BottomStrand><ColorRange range="1900..1900" colors="red"/><ColorRange range="2044..2044" colors="red"/><ColorRange range="2125..2125" colors="red"/><ColorRange range="2156..2179" colors="blue"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
